Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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Separation was carried out at room temperature. Antivacterial Pacific Journal of Tropical Biomedicine. The combined chloroform extract was washed with water, dipped, and dried over anhydrous sodium sulphate.

In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

The leaves are used to treat malarial fever, chronic fever, intrinsic hemorrhage, cough, asthma, leprosy, skin diseases, and piles [ 6 ].

Antimicrobial Activity Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ]. Natibacterial1 C. Natural products still remain the most important source for discovery of new and potential drug molecules.

Mobile phase A consisted of water with 0. Adhatoda vasica, Bauhenia variegate, Bombax ceiba, Carrisa opaca, Caryopteris grata, Debregeasia salicifolia, Lantana camara, Melia azedarach, Phyllanthus emblica, Pinus roxburghii and Olea ferruginea collected from lower Himalayas against two Gram positive Staphylococcus aureus, Micrococcus luteus and two Gram negative Escherichia coli, Pseudomonas aureginosa bacterial strains.


It was then distilled on a water bath and the residue was washed with little n-hexane. Results and Discussion The ethanol extract of A.

Classical Uses of Medicinal Plants. The free radical scavenging activity was calculated as follows:. A voucher specimen No.

The crude extract of A.

The physical and spectroscopic data were compared with those reported in the literature [ 15 ]. Indian Journal of Microbiology. Table 2 Minimum inhibitory concentration of Vasicine acetate against tested bacteria and fungi.

Two mobile phases A and B were used at flow rate of 1. Achatoda structure was elucidated using spectroscopic methods: Vasicine acetate was obtained by acetylation of Vasicine. The plant was identified by Dr. The authors declare that there is no conflict of interests regarding the publication of this paper.

Table 4 Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line.

Antibacterial activity of adhatoda vasica leaf extract

International Journal of Green Pharmacy. Structure of the compounds: Support Center Support Center. The spot turned orange red on spraying with Dragendorff’s reagent.

Minimum inhibitory concentration of Vasicine acetate against tested bacteria and fungi. Journal of Natural Products.

Antibacterial Activity of Leaf Extracts of Adhatoda vasica – MAHE Digital Repository

The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A. Conflict of Interests The authors declare that adhafoda is no conflict of interests regarding the publication of this paper. Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].


Journal of Natural Product and Plant Resources. Medicinal plants are important sources of practical drugs for people throughout the year.

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Open in a separate window. Ignacimuthu and Shanmugam [ 15 ] have reported the antitubercular activity of Vasicine acetate isolated from A. It is inferred that methanolic crude extracts of the above mentioned plantsexhibitantibacterial activities against pathogenic bacteria, which proved the ethnobotanical importance of the selected plants that indigenous people use for cure against various diseases.

The MIC for fungi was defined as the lowest extract concentration, showing no visible fungal growth after incubation time. Antibacterial activities against Adhztoda aureus and Micrococcus luteus were observed formethanolic extracts of all the above mentioned plants.